INGUBAL Liquid Granja® postbiotic for colibacillosis control in a poultry farm
Ingubal Liquid Granja


Colibacillosis is an infectious disease caused by avian pathogenic Escherichia coli (APEC) that causes significant economic losses in the poultry sector. The emergence of multi-resistant strains to antibiotics and the restriction of animal feed additives has increased the need for the development of alternatives to help control this disease. The postbiotic
INGUBAL Liquid Farm
® for the control of colibacillosis in a poultry farm is an innovative product containing beneficial strains and the antimicrobial and immunomodulatory metabolites produced by these strains. In our study we elaborated
INGUBAL Liquid Farm
® was developed to reduce the prevalence of APEC in a poultry farm and improve its profitability thanks to an increase in production parameters and a decrease in the prescription of antibiotics.



A clinical study was conducted with the postbiotic
INGUBAL Líquid Granja
® for the control of colibacillosis in a poultry farm. Medical history data were collected regarding mortality rates and previous epidemiological studies. Isolation, identification and molecular characterization of bacterial pathogens was carried out with samples obtained from bedding, as well as from clinical cases reported from different houses of the farm. Enteropathogenic E.coli (APEC) strains were isolated as the causal agent of colibacillosis, responsible for high mortality rates and production losses on the farm. Two houses of 15,000 chicks each were selected. Vessel A was the control group and vessel B received the postbiotic.
INGUBAL Líquid Granja
® in suspension in the drinking water during the entire 45-day production cycle at a concentration of 0.1% three days per week.

Different periodic sampling of bedding with fecal content was performed to analyze the proportion of APEC strains(E. coli with two or more pathogenicity factors fimC, iucD, papC). A macroscopic anatomopathological study was performed, as well as a microbiological analysis of the animals that died during the study to determine the most probable cause of death. At the end of the study, the mortality rate was calculated and the animals and feed were weighed to estimate the productive parameters.



A higher proportion of APEC strains was found in bedding samples from the control house compared to the supplemented house (Table 1), indicating a higher intestinal bacterial load of the pathogen in the animals of the control group.

Table 1-Ingubal Liquid Farm ResultsTable 1. Percentage of APEC-positive samples in broiler litter

In addition, the cause of death in the control group animals was mostly APEC infection, confirmed both by the macroscopic lesions observed during necropsy of the animals (Figure 1) and by the biochemical and molecular confirmation of the strains isolated from them. It is noteworthy that in these cases the E. coli strain isolated was multidrug resistant to several groups of antibiotics (Table 2). This pathogen was not detected in any dead animal of the supplemented group.

Table 2 - Ingubla Liquid Farm ResultsTable 2. Results of antimicrobial susceptibility testing of isolates from a clinical case of vessel A (control).

As a result, antibiotherapy was prescribed in the control group 2.2 times more than in the supplemented group.

Figure 1. Fibrinous pericarditis (A), fibrinopurulent pericarditis (B), polyserositis (C) and arthritis (D) associated with colibacillosis caused by avian pathogenic E. coli (APEC).

Figure 1. Fibrinous pericarditis (A), fibrinopurulent pericarditis (B), polyserositis (C) and arthritis (D) associated with colibacillosis caused by avian pathogenic E. coli (APEC).

As a result of all of the above, the productive parameters improved notably in the supplemented group (Table 3). A significantly lower mortality rate, higher average weight and slightly lower feed conversion rate were obtained in the group that consumed postbiotic.

Table 3. Productive parameters of both study groups.



Avian pathogenic Escherichia coli (APEC) is a pathogenic bacterium with a high proportion of pathogenicity factors that has the potential to cause colibacillosis, an important disease that causes significant health and economic losses in poultry.
has been specifically designed to help control this disease by studying the causal pathogens.

In chickens supplemented throughout the production cycle, a decrease in the intestinal load of APEC strains resulted in a lower prevalence of colibacillosis, since oro-fecal transmission is the main route of infection. Postbiotics may also contribute to the maintenance of a healthy microbiota and thus to intestinal barrier functions that prevent colonization of pathogenic bacteria and enhance nutrient synthesis and absorption. Improved production parameters in supplemented flocks are related to better health indicators and optimal nutrient utilization. In addition to the significant improvement in production parameters (72.4% reduction in mortality due to colibacillosis and 0.5% reduction in the Conversion Index), reduced antibiotic prescribing also contributes to improved farm profitability and helps control the global public health problem of antimicrobial resistance.



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This study has been carried out by INGULADOS in collaboration with Grupo VMR.